99.8 99.9L 100.0 0.02 0.03% 0.04 21.0 21.2% 21.4 200 0 km 200 Phanerozoic Eons Cenozoic Eras Neogene Periods Miocene Pliocene PLE Epochs BUR Langhian SER Tortonian Messinian Zanclean PIA Gelasian Lower Middle Ages 0.54 1.54 2.55 3.55 4.55 5.55 6.56 7.56 8.56 9.56 10.57 11.57 12.57 13.57 14.58 15.58 16.58 17.59 18.59 19.59 MYA axolotl Ambystoma mexicanum Versus tiger salamander Ambystoma tigrinum Median Time: 8.2 MYA CI: (5.1 - 11.2 MYA) Due to conflicting time estimates between studies, ancestral nodes can be assigned younger ages than their descendants. A smoothing technique is used to adjust these times so the resulting tree is ultrametric.Adjusted Time: 8.2 MYA Kumar S, Stecher G, Suleski M, Hedges SB (2017). TimeTree: A Resource for Timelines, Timetrees, and Divergence Times. Mol Biol Evol 34:1812-1819citation (Median and adjusted times were derived from 7 studies)